Immunohistochemistry (IHC)

Lymohoma/Leukemia typing. CD30 is a lymphocyte activation antigen, related to tumor necrosis factor. Positive staining (normal): granulocytes, plasma cells, activated B-, T- and NK cells. Positive staining (disease): infectious mononucleosis, lymphocytes infected with HIV, HTLV-1, EBV, HHV8 or hepatitis B; Reed-Sternberg cells, 90% of anaplastic large cell lymphomas, lymphomatoid papulosis, peripheral T-cell lymphomas, germ cell tumors and some melanomas.

One (1) formalin-fixed, paraffin-embedded (FFPE) unbaked, unstained slide cut at 4-5 microns for H&E staining (required) and three (3) positively charged unstained slides cut at 3-4 microns for each test/antibody ordered

or

A formalin-fixed, paraffin-embedded (FFPE) tissue block All blocks and slides must have two (2) identifiers clearly written and match exactly with the specimen identifies and specimen labeling on accompanying req.

Use cold pack for transport. Cold pack shouldn’t come in direct contact with specimen.

88342

Global

Tech Only 

24-48 Hours

Mismatch repair (MMR) genes results in failure to repair errors in repetitive sequences that occur during DNA replication. The defects in DNA repair pathways have been related to tumor carcinogenesis. Studies have shown the mutations of MLH-1, MSH2 and MSH6 genes contribute to the development of sporadic colorectal carcinoma. MSH6 is a heterodimer of MSH2 and binds to DNA containing G/T mismatches. Germ-line mutations of MLH1 and MSH2 account for 90% of all known MMR mutations in HNPCC and mutation of MSH6 account for another 5-10%, whereas mutations of other genes are rare.

One (1) formalin-fixed, paraffin-embedded (FFPE) unbaked, unstained slide cut at 4-5 microns for H&E staining (required) and three (3) positively charged unstained slides cut at 3-4 microns for each test/antibody ordered

or

A formalin-fixed, paraffin-embedded (FFPE) tissue block All blocks and slides must have two (2) identifiers clearly written and match exactly with the specimen identifier and specimen labeling on accompanying req.

Use cold pack for transport. Cold pack shouldn’t come in direct contact with specimen.

88342

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24-48 Hours

CK20 positivity is seen in the majority of adenocarcinomas of the colon, mucinous ovarian carcinomas, transitional cell and Merkel cell carcinomas and frequently in adenocarcinomas of the stomach, bile system and pancreas. The primary sites of adenocarcinoma causing the most cancer deaths are the lung and colon. However, pathological differentiation of these two neoplasms can be difficult. Cytokeratin 7 is usually present in pulmonary but not colonic adenocarcinomas. CK7/CK20 immunostaining patterns may be helpful in separating pulmonary from colonic adenocarcinomas.

One (1) formalin-fixed, paraffin-embedded (FFPE) unbaked, unstained slide cut
at 4-5 microns for H&E staining (required) and three (3) positively charged unstained slides cut at 3-4 microns for each test/antibody ordered

or

A formalin-fixed, paraffin-embedded (FFPE) tissue block All blocks and slides must have two (2) identifiers clearly written and match exactly with the specimen identifies and specimen labeling on accompanying req.

Use cold pack for transport. Cold pack shouldn’t come in direct contact with specimen.

88342

Global 

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24-48 Hours

The product of the p53 gene is a nuclear phosphoprotein that regulates cell proliferation. Excess accumulation of the mutant p53 gene product results in inactivation of its tumor suppressor function and cellular transformation. Overexpression of mutant p53 gene has also been associated with high proliferative rates and poor prognosis in breast, colon, lung, and brain cancer, as well as in some leukemias and lymphomas.

One (1) formalin-fixed, paraffin-embedded (FFPE) unbaked, unstained slide cut at 4-5 microns for H&E staining (required) and three (3) positively charged unstained slides cut at 3-4 microns for each test/antibody ordered

or

A formalin-fixed, paraffin-embedded (FFPE) tissue block All blocks and slides must have two (2) identifiers clearly written and match exactly with the specimen identifier and specimen labeling on accompanying req.

Use cold pack for transport. Cold pack shouldn’t come in direct contact with specimen.

88342, 88361

Global

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24-48 Hours

E-Cadherin is an adhesion protein that is expressed in cells of epithelial lineage.
It stains positively in glandular epithelium as well as adenocarcinomas of the lung and G.I. tract and ovary. It is useful in distinguishing adenocarcinoma from mesothelioma. It has also been shown to be positive in some thyroid carcinomas. Breast carcinomas with ductal and lobular features show two staining patterns: (1) complete or almost complete lack of membrane staining in lobular carcinomas and (2) uniform membrane expression throughout the tumor in ductal carcinomas. Immunohistochemical detection of E-Cadherin expression can be a useful diagnostic tool for the differentiation of ductal and lobular carcinomas of the breast.

One (1) formalin-fixed, paraffin-embedded (FFPE) unbaked, unstained slide cut at 4-5 microns for H&E staining (required) and three (3) positively charged unstained slides cut at 3-4 microns for each test/antibody ordered

or

A formalin-fixed, paraffin-embedded (FFPE) tissue block All blocks and slides must have two (2) identifiers clearly written and match exactly with the specimen identifier and specimen labeling on accompanying req.

Use cold pack for transport. Cold pack shouldn’t come in direct contact with specimen.

88342

Global

Tech Only

24-48 Hours 

p63 is a homologue of the p53 gene and is necessary for normal breast and prostate development. Unlike other markers of myoepithelial cells and basal cells, p63 immunoreactivity is localized to the nucleus of the cells, which can offer distinct advantages over cytoplasmic labeling in certain types of cases. P63, as a marker of myoepithelial and basal cells, is extremely useful in diagnostic surgical pathology, particularly when examining difficult breast biopsies and prostate biopsies.

One (1) formalin-fixed, paraffin-embedded (FFPE) unbaked, unstained slide cut at 4-5 microns for H&E staining (required) and three (3) positively charged unstained slides cut at 3-4 microns for each test/antibody ordered

or

A formalin-fixed, paraffin-embedded (FFPE) tissue block All blocks and slides must have two (2) identifiers clearly written and match exactly with the specimen identifier and specimen labeling on accompanying req.

Use cold pack for transport. Cold pack shouldn’t come in direct contact with specimen.

88342

Global

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24-48 Hours

Anti-ER (SP1) is directed against an epitope present on human ER protein located in the
nucleus of ER positive normal and neoplastic cells. Anti-ER (SP1) is indicated as an aid in the management, prognosis, and prediction of therapy outcome of breast cancer

One (1) formalin-fixed, paraffin-embedded (FFPE) unbaked, unstained slide cut at 4-5 microns for H&E staining (required) and three (3) positively charged unstained slides cut at 3-4 microns for each test/antibody ordered

or

 A formalin-fixed, paraffin-embedded (FFPE) tissue block All blocks and slides must have two (2) identifiers clearly written and match exactly with the specimen identifier and specimen labeling on accompanying req.

Use cold pack for transport. Cold pack shouldn’t come in direct contact with specimen.

88360

Global

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24-48 Hours

In IHC, P504S has been shown to be a valuable marker of prostatic adenocarcinoma.
Additionally, prostate glands involved in PIN have been found to express P504S, whereas P504S was nearly undetectable in benign glands.

One (1) formalin-fixed, paraffin-embedded (FFPE) unbaked, unstained slide cut at 4-5 microns for H&E staining (required) and three (3) positively charged unstained slides cut at 3-4 microns for each test/antibody ordered

or

A formalin-fixed, paraffin-embedded (FFPE) tissue block All blocks and slides must have two (2) identifiers clearly written and match exactly with the specimen identifier and specimen labeling on accompanying req.

Use cold pack for transport. Cold pack shouldn’t come in direct contact with specimen.

88344

Global

Tech Only

24-48 Hours

The HER2 oncogene is over-expressed in some breast carcinomas. The expected over-expression rate varies based on the grade and type of breast cancer. Tumors showing 3+ over-expression of HER2 may benefit from trastuzumab therapy. Borderline results (2+) show a significantly reduced response rate to trastuzumab therapy.
Assessment of the HER2 gene status may provide additional therapeutic information
in some cases. Known artifacts such as edge artifact, tissue retraction and tissue crush may give the false impression of over-expression. Care should be taken to avoid assessing these areas, especially in needle core biopsies that generally harbor all of these artifacts.

One (1) formalin-fixed, paraffin-embedded (FFPE) unbaked, unstained slide cut at 4-5 microns for H&E staining (required) and three (3) positively charged unstained slides cut at 3-4 microns for each test/antibody ordered

or

A formalin-fixed, paraffin-embedded (FFPE) tissue block All blocks and slides must have two (2) identifiers clearly written and match exactly with the specimen identifier and specimen labeling on accompanying req.

Use cold pack for transport. Cold pack shouldn’t come in direct contact with specimen.

88360

Global

Tech Only

24-48 Hours

PD-L1 IHC 22C3 pharm Dx is the only companion diagnostic indicated as an aid in identifying patients with NSCLC for treatment with KEYTRUDA® (pembrolizumab)

One (1) formalin-fixed, paraffin-embedded (FFPE) unbaked, unstained slide cut at 4-5 microns for H&E staining (required) and three (3) positively charged unstained slides cut at 3-4 microns for each test/antibody ordered

or

A formalin-fixed, paraffin-embedded (FFPE) tissue block All blocks and slides must have two (2) identifiers clearly written and match exactly with the specimen identifier and specimen labeling on accompanying req.

Use cold pack for transport. Cold pack shouldn’t come in direct contact with specimen.

88360

Global

Tech Only

24-48 Hours

Ki-67 is a nuclear protein that is expressed in proliferating cells. Ki-67 is preferentially expressed during late G1-, S-, M-, and G2-phases of the cell cycle, while cells in the G0 (quiescent) phase are negative for this protein. Increased proliferative activity is associated with more aggressive tumor and decreased disease-free survival period.

One (1) formalin-fixed, paraffin-embedded (FFPE) unbaked, unstained slide cut at 4-5 microns for H&E staining (required) and three (3) positively charged unstained slides cut at 3-4 microns for each test/antibody ordered

or

A formalin-fixed, paraffin-embedded (FFPE) tissue block All blocks and slides must have two (2) identifiers clearly written and match exactly with the specimen identifier and specimen labeling on accompanying req.

Use cold pack for transport. Cold pack shouldn’t come in direct contact with specimen.

88342, 88361

Global 

Tech Only

24-48 Hours

The VENTANA PD-L1 (SP142) Assay is a qualitative immunohistochemical assay using rabbit monoclonal anti-PD-L1 clone SP142 intended for use in the assessment of the PD-L1 protein in formalin-fixed, paraffin-embedded (FFPE) urothelial carcinoma and non-small cell lung cancer (NSCLC) tissue on a VENTANA BenchMark ULTRA instrument. Determination of PD-L1 status is indication-specific, and evaluation is based on either the proportion of tumor area occupied by PD-L1 expressing tumor-infiltrating immune cells (% IC) of any intensity or the percentage of PD-L1 expressing tumor cells (% TC) of any intensity. Primary or metastatic urothelial carcinoma (bladder cancer) or NSCLC (lung cancer) tissues may be submitted.

One (1) formalin-fixed, paraffin-embedded (FFPE) unbaked, unstained slide cut at 4-5 microns for H&E staining (required) and three (3) positively charged unstained slides cut at 3-4 microns for each test/antibody ordered

or

A formalin-fixed, paraffin-embedded (FFPE) tissue block All blocks and slides must have two (2) identifiers clearly written and match exactly with the specimen identifier and specimen labeling on accompanying req.

Use cold pack for transport. Cold pack shouldn’t come in direct contact with specimen.

88360

Global 

Tech Only

24-48 Hours

MART-1 (Melanoma Antigen Recognized by T cells 1) recognizes a protein of 18 kDa, a
subcellular fraction found in melanosomes. The antibody labels melanomas and tumors showing melanocytic differentiation. It does not mark neoplasms of epithelial origin, lymphomas or mesenchymal tumors.

One (1) formalin-fixed, paraffin-embedded (FFPE) unbaked, unstained slide cut at 4-5 microns for H&E staining (required) and three (3) positively charged unstained slides cut at 3-4 microns for each test/antibody ordered

or

A formalin-fixed, paraffin-embedded (FFPE) tissue block All blocks and slides must have two (2) identifiers clearly written and match exactly with the specimen identifier and specimen labeling on accompanying req.

Use cold pack for transport. Cold pack shouldn’t come in direct contact with specimen.

88342

Global

Tech only

24-48 Hours

Mismatch repair (MMR) genes result in failure to repair errors in repetitive sequences that occur during DNA replication. This failure leads to microsatellite instability (MSI) of the tumor, which is the hallmark of HNPCC. Increased risk for malignancy in HNPCC is caused by a mutation in one of the following DNA mismatch repair (MMR) genes; MLH1, MSH2, MSH3, MSH6, PMS1, and PMS2. Germ-line mutations of MLH1 and MSH2 account for 90% of all known MMR mutations in HNPCC and mutation of MSH6 account for another 5-10%, whereas mutations of other genes are rare. PMS2 protein forms a heterodimer with the MLH1 protein. Due to this, the absence of the MLH1 protein due to germ-line mutation also leads to loss of PMS2 protein.

One (1) formalin-fixed, paraffin-embedded (FFPE) unbaked, unstained slide cut at 4-5 microns for H&E staining (required) and three (3) positively charged unstained slides cut at 3-4 microns for each test/antibody ordered

or

A formalin-fixed, paraffin-embedded (FFPE) tissue block All blocks and slides must have two (2) identifiers clearly written and match exactly with the specimen identifier and specimen labeling on accompanying req.

Use cold pack for transport. Cold pack shouldn’t come in direct contact with specimen.

88342

Global

Tech Only

24-48 Hours

Mismatch repair genes perform an essential cellular function by repairing DNA mismatches that may occur during cellular replication. A number of these genes have been identified in human genome, including hMLH1, hMSH2, hMSH3, hMSH6, hPMS1, and hPMS2. MLH1 and MSH2 proteins are normally expressed in the nucleus of cells. The absence of nuclear expression of one or both of these proteins has been found to correlate with the presence of a mismatch repair gene defect in the respective gene.
Recent studies have been found that 50-70% of patients with hereditary non-polyposis colorectal cancer syndrome (HNPCC) have a deficient DNA mismatch repair. Patients with HNPCC have an 80-90% lifetime risk of colorectal carcinoma, and typically have an earlier onset (mean age of onset 42: vs. 65 years for conventional colon cancer).

One (1) formalin-fixed, paraffin-embedded (FFPE) unbaked, unstained slide cut at 4-5 microns for H&E staining (required) and three (3) positively charged unstained slides cut at 3-4 microns for each test/antibody ordered

or

A formalin-fixed, paraffin-embedded (FFPE) tissue block All blocks and slides must have two (2) identifiers clearly written and match exactly with the specimen identifier and specimen labeling on accompanying req.

Use cold pack for transport. Cold pack shouldn’t come in direct contact with specimen.

88342

Global 

Tech Only 

24-48 Hours

Anti-PR (1E2) Primary Antibody is a rabbit monoclonal antibody (IgG) that is intended for laboratory use for the qualitative detection of progesterone receptor (PR) antigen in sections of formalin fixed, paraffin embedded tissue.

One (1) formalin-fixed, paraffin-embedded (FFPE) unbaked, unstained slide cut at 4-5 microns for H&E staining (required) and three (3) positively charged unstained slides cut at 3-4 microns for each test/antibody ordered

or

A formalin-fixed, paraffin-embedded (FFPE) tissue block All blocks and slides must have two (2) identifiers clearly written and match exactly with the specimen identifier and specimen labeling on accompanying req.

Use cold pack for transport. Cold pack shouldn’t come in direct contact with specimen.

88360, 88361

Global

Tech Only

24-48 Hours

Mismatch repair genes perform an essential cellular function by repairing DNA mismatches that may occur during cellular replication. A number of these genes have been identified in human genome, including hMLH1, hMSH2, hMSH3, hMSH6, hPMS1, and hPMS2. MLH1 and MSH2 proteins are normally expressed in the nucleus of cells. The absence of nuclear expression of one or both of these proteins has been found to correlate with the presence of a mismatch repair gene defect in the respective gene.
Recent studies have been found that 50-70% of patients with hereditary non-polyposis colorectal cancer syndrome (HNPCC) have a deficient DNA mismatch repair. Patients with HNPCC have an 80-90% lifetime risk of colorectal carcinoma, and typically have an earlier onset (mean age of onset: 42 vs. 65 years for conventional colon cancer).

One (1) formalin-fixed, paraffin-embedded (FFPE) unbaked, unstained slide cut at 4-5 microns for H&E staining (required) and three (3) positively charged unstained slides cut at 3-4 microns for each test/antibody ordered

or
A formalin-fixed, paraffin-embedded (FFPE) tissue block
All blocks and slides must have two (2) identifiers clearly written and match exactly with the specimen identifier and specimen labeling on accompanying req.

Use cold pack for transport. Cold pack shouldn’t come in direct contact with specimen.

88342

Global

Tech Only

24-48 Hours

SOX10 is a sensitive marker of melanoma, including conventional, spindled, and desmoplastic subtypes. It is also a useful marker in detecting both the in situ and invasive components of desmoplastic melanoma. SOX10 is diffusely expressed in schwannoma, neurofibroma, and granular cell tumor. SOX10 was not identified in any other mesenchymal and epithelial tumors except for myoepitheliomas and diffuse astrocytomas.

One (1) formalin-fixed, paraffin-embedded (FFPE) unbaked, unstained slide cut at 4-5 microns for H&E staining (required) and three (3) positively charged unstained slides cut at 3-4 microns for each test/antibody ordered

or

A formalin-fixed, paraffin-embedded (FFPE) tissue block All blocks and slides must have two (2) identifiers clearly written and match exactly with the specimen identifier and specimen labeling on accompanying req.

Use cold pack for transport. Cold pack shouldn’t come in direct contact with specimen.

88342

Global

Tech Only

24-48 Hours