Cytopathology

Preparation of slide(s) from submitted urine sample utilizing liquid based techniques to include ThinPrep and cytospin for microscopic interpretation. Clinical Significance, utilized for the initial  detection or recurrence of high grade urothelial carcinoma
 

Minimum 30-40 ml of voided (second-morning void) or catherized urine; instrumented samples to include washings or brushings of bladder, urethra, ureters or renal pelvis. Additional 33 ml of urine required for Bladder Cancer FISH profile- UroVysion®
All jars must be labeled with two (2) patient identifiers clearly written and matching exactly with the specimen identifiers and specimen labeling on the accompanying requisition

Sterile plastic container
 Ambient, are to be refrigerated after 1hr of collection.  If delay of more than 24 Hours is expected add an equal amount of ethanol (50%) or Cytolyt to the sample (urine samples for microbiology/culture are to remain unfixed/fresh). 
Use cold pack for transport. Cold pack shouldn’t come in direct contact with specimen.

88112, 8811226

Global

48-72 hours for all (fluid/brushing, Urine and FNA cytology specimens)

Preparation of slide(s) from submitted FNA sample utilizing either liquid based techniques to include ThinPrep and/or cytospin methodologies and/or review of submitted direct smears for microscopic interpretation. Preparation and review of Cell Block material as needed. Clinical Significance utilized for the detection of normal/benign and reactive, infectious and/or inflammatory processes and identification of atypical, premalignant and malignant entities in cellular samples

Liquid based preparation: 2-4 FNA passes into 30ml prefilled Cytolyt tube per nodule/site
Direct Smear Collection: Direct smears immediately fixed in 95% ethanol for Papanicolaou (PAP) staining. Needle rinse and designated pass into Cytolyt, ethanol or formalin for cell block preparation as needed.
Diff-Quik staining (if needed): Air-dried direct smears
For specimens in which a hematopoietic disorder cannot be excluded or Lymphoma is suspected additional FNA material to be collected in RPMI for flow cytometry testing All jars, tubes and slides must be labeled with two (2) patient identifiers clearly written and matching exactly with the specimen identifiers and specimen labeling on the accompanying requisition

30 ml Cytolyt tube or 1-2, Air-dried direct smears in cardboard holder And/or 2-4, Direct smears (ethanol fixed) in coplin jar

And 15ml Cytolyt, ethanol or formalin jar for needle rinse/cellblock
Ambient, refrigerated. Use cold pack for transport if necessary.

Cold pack shouldn’t come in direct contact with specimen.

88173, 88313, 88305, 88172, 88177, 88321

Global

Tech Only

Professional Only

48-72 hours for all (fluid/brushing, Urine and FNA cytology specimens)

Targeted mutation detention by next generation sequencing in Thyroid (FNA) and tissue
samples, Thyroseq v3 next generation sequencing panel offers simultaneous sequencing and detention in >1000 hotspots of 14 thyroid cancer-related genes and for 42 types of gene fusions known to occur in thyroid cancer.

Clinical Significance
 

ThyroSeq® Genomic Classifier (GC) is a test for the pre-operative assessment of thyroid nodules with indeterminate cytology, which offers accurate assessment of cancer probability in a given nodule and additionally provides information on cancer prognostication, helping to select the most optimal patient management.
ThyroSeq incorporates all major scientific advances in thyroid cancer genetics and has more than 10-years experience serving physicians and their patients with thyroid nodules and cancer. The first version of ThyroSeq was launched for clinical use at the University of Pittsburgh Medical Center as a seven-gene panel (ThyroSeq v0) in April of 2007. Until recently, the test was offered as ThyroSeq v3. Today, ThyroSeq v3 is available for clinical use.

FNA is the primary sample type for ThyroSeq testing and is a dedicated fresh FNA collected into ThyroSeqPreserve solution. This sample type has the highest sample success rate (96%) and shortest turnaround time. If a fresh FNA sample collected into ThyroSeqPreserve is not available or has failed ThyroSeq testing, cytology smears can be submitted for testing.
 For cytology smear sample submission: 1-2 slides with at least 200-300 cells are required. Diff-Quik® and Papanicolau (Pap) stained smears are acceptable
                If the above conditions are met, there is a 79-93% success rate of testing.
                An increased turnaround time by 2-3 days should be expected for processing of
                cytology smears.

NOTE: Cytology slides will be destroyed during molecular testing and cannot be returned. It is up to the sending institution to save digital images of the slides prior to sending for testing.
FNA Cell Block: Submit two H&E slides and 10 unstained slides.
Resected Tumors - FFPE Tissue Specimens-1 H&E and 6 unstained slides containing at least 3 mm of tumor cut at 4-5 microns.

Shipping Conditions: After the sample is collected, the specimen should be kept at -20º C. If this is not possible, the specimen can be kept at room temperature for no longer than 6 hours or at 4ºC for no longer than 24 hours. Specimens can be shipped at room temperature when using “next business morning” delivery or with ice packs by overnight mail.

Varies by payer

Global

7-10 days