The Acute Myeloid Leukemia (AML) Panel by flow cytometry is used to characterize the immunophenotype of a myeloblast population to aid in the diagnosis and subclassification of AML and other myeloid neoplasms.
This panel is best used in combination with the Leukemia/Lymphoma Panel.

Bone marrow aspirate: 2-3 ml in green top (sodium heparin) or purple top (EDTA) tube
Peripheral blood: 5-7 ml in green top (sodium heparin) or purple top (EDTA) tube

Specimens should be received within 24-48 Hours from collection to ensure acceptable cell viability. Peripheral blood and bone marrow aspirate specimens can be stored and transported at room temperature. Other tissue biopsies and body fluids should be refrigerated but not frozen; please use a cold pack to transport these specimens, making sure the cold pack is not in direct contact with the specimen.

+88184, 88185, 88189

DNA stain propidium iodide is used to determine S-phase cell cycle fraction and DNA index as indicators of DNA ploidy. Clinical Significance DNA analysis is, after immunofluorescence, the second most important application of flow cytometry. By measuring the DNA content of individual cell, we can obtain information about their ploidy, of particular relevance in tumors, and, for a population, the distribution of cells across the cell cycle.

Flow cytometry testing can be performed on bone marrow aspirate, peripheral
blood, fresh bone marrow core biopsy, unfixed tissue, and body fluids.
Please see full specimen requirements for either Standard Leukemia/Lymphoma
Analysis or Extended Leukemia/Lymphoma Analysis.

Specimens should be received within 24-48 Hours from collection to ensure acceptable cell viability. Peripheral blood and bone marrow aspirate specimens can be stored and transported at room temperature. Other tissue biopsies and body fluids should be refrigerated but not frozen; please use a cold pack to transport these specimens, making sure the cold pack is not in direct contact with the specimen.

 

The Hairy Cell Leukemia Panel by flow cytometry is used to aid in the initial diagnosis of hairy cell leukemia or hairy cell leukemia variant or to assess for the presence of residual/relapsed disease. This panel is best used in combination with the Leukemia/Lymphoma Panel.

Bone marrow aspirate: 2-3 ml in green top (sodium heparin) or purple top (EDTA) tube
Peripheral blood: 5-7 ml in green top (sodium heparin) or purple top (EDTA) tube

Specimens should be received within 24-48 Hours from collection to ensure acceptable cell viability. Peripheral blood and bone marrow aspirate specimens can be stored and transported at room temperature. Other tissue biopsies and body fluids should be refrigerated but not frozen; please use a cold pack to transport these specimens, making sure the cold pack is not in direct contact with the specimen.

+88184, 88185, 88187

Clinical Significance
The Immune Deficiency Panel by flow cytometry is used to quantify the relative proportions and absolute numbers of T, B, and NK cells in the peripheral blood. This data can help to evaluate the status of a patient’s immune system and assess degree of immunodeficiency, for example, by measuring absolute CD4 counts in patients with HIV infection.

Peripheral blood: 5-7 ml in purple top (EDTA) tube (preferred) or green top (sodium heparin) tube

Specimens should be received within 24 Hours from collection to ensure sample integrity and acceptable cell viability. Specimens should be transported at room temperature.

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12-24 Hours

This is a general panel used to screen peripheral blood or bone marrow specimens for the presence of hematolymphoid neoplasms.

Bone marrow aspirate: 2-3 ml in green top (sodium heparin) or purple top (EDTA) tube
Peripheral blood: 5-7 ml in green top (sodium heparin) or purple top (EDTA) tube

Specimens should be received within 24-48 Hours from collection to ensure sample integrity and acceptable cell viability. Specimens should be stored and transported at room temperature.

+88184, 88185, 88189

24-36 Hours

This is a general panel used to screen lymph node and other tissue biopsies for the presence of lymphoproliferative disorders.

Incisional/excisional or core needle biopsy: Ideally, 1 cm3 of tissue completely immersed in RPMI. Testing can be successfully performed on smaller specimens if the tissue has adequate cellularity. The number of markers tested may be reduced if cellularity is insufficient. If RPMI is unavailable, tissue can be submitted wrapped in saline-moistened gauze but not submerged in saline.
Fine needle aspirate (FNA): 1:1 ratio of aspirate and RPMI; minimum 2 ml total

Specimens should be received within 24-48 Hours from collection to ensure sample integrity and acceptable cell viability. Specimens should be refrigerated but not frozen. Please use a cold pack for transport, making sure the cold pack is not in direct contact with the specimen

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The PNH Panel by flow cytometry is used to diagnose and monitor patients with paroxysmal nocturnal hemoglobinuria (PNH).

Peripheral blood: 3 ml in purple top (EDTA) tube

Specimens should be received within 24 hours of draw time to ensure sample integrity and acceptable cell viability.

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The Plasma Cell Neoplasm Panel by flow cytometry is used to aid in the initial diagnosis of plasma cell neoplasms (i.e., plasma cell myeloma, plasmacytomas, and monoclonal gammopathy of undetermined significance) or to assess for the presence of residual/relapsed disease. This panel is best used in combination with the Leukemia/Lymphoma Panel.

Bone marrow aspirate: 2-3 ml in green top (sodium heparin) or purple top (EDTA) tube
Peripheral blood: 5-7 ml in green top (sodium heparin) or purple top (EDTA) tube Incisional/excisional or core needle biopsy: Ideally, 1 cm3 of tissue completely immersed in RPMI. Testing can be successfully performed on smaller specimens if the tissue has adequate cellularity. If RPMI is unavailable, tissue can be submitted wrapped in saline-moistened gauze but not submerged in saline.
Fine needle aspirate (FNA): 1:1 ratio of aspirate and RPMI; minimum 2 ml total

Specimens should be received within 24-48 hours from collection to ensure
sample integrity and acceptable cell viability. Specimens should be stored and transported at room temperature.

+88184, 88185, 88187

The T-Cell Panel by flow cytometry is used to aid in the diagnosis of T-cell leukemias/lymphomas. This panel is best used in combination with the Leukemia/ Lymphoma Panel.

Bone marrow aspirate: 2-3 ml in green top (sodium heparin) or purple top (EDTA) tube
Peripheral blood: 5-7 ml in green top (sodium heparin) or purple top (EDTA) tube Incisional/excisional or core needle biopsy: Ideally, 1 cm3 of tissue completely immersed in RPMI. Testing can be successfully performed on smaller specimens if the tissue has adequate cellularity. If RPMI is unavailable, tissue can be submitted wrapped in saline-moistened gauze but not submerged in saline.
Fine needle aspirate (FNA): 1:1 ratio of aspirate and RPMI; minimum 2 ml total

Specimens should be received within 24-48 Hours from collection to ensure
sample integrity and acceptable cell viability.

Peripheral blood and bone marrow aspirate specimens should be stored and transported at room temperature.

Other tissue biopsies and body fluids should be refrigerated but not frozen; please use a cold pack to transport these specimens, making sure the cold pack is not in direct contact with the specimen.

+88184, 88185, 88187

Clinical Significance: The ZAP-70 Panel by flow cytometry is used to assess for the presence of ZAP-70 expression on chronic lymphocytic leukemia/small lymphocytic lymphoma. The presence of ZAP-70 expression has been associated with an adverse prognosis and correlates with unmutated IGHV status. This panel is best used in combination with the Leukemia/Lymphoma Panel.

Peripheral blood: 5-7 ml in green top (sodium heparin) or purple top (EDTA) tube

Specimens should be received within 24 hours of draw time.
Specimens should be stored and transported at room temperature.

+88184, 88185, 88187